APOBEC3G-UBA2 fusion as a potential strategy for stable expression of APOBEC3G and inhibition of HIV-1 replication
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منابع مشابه
Deaminase-independent inhibition of HIV-1 reverse transcription by APOBEC3G
APOBEC3G (A3G), a host protein that inhibits HIV-1 reverse transcription and replication in the absence of Vif, displays cytidine deaminase and single-stranded (ss) nucleic acid binding activities. HIV-1 nucleocapsid protein (NC) also binds nucleic acids and has a unique property, nucleic acid chaperone activity, which is crucial for efficient reverse transcription. Here we report the interplay...
متن کاملThe Roles of APOBEC3G Complexes in the Incorporation of APOBEC3G into HIV-1
BACKGROUND The incorporation of human APOBEC3G (hA3G) into HIV is required for exerting its antiviral activity, therefore the mechanism underlying hA3G virion encapsidation has been investigated extensively. hA3G was shown to form low-molecular-mass (LMM) and high-molecular-mass (HMM) complexes. The function of different forms of hA3G in its viral incorporation remains unclear. METHODOLOGY/PR...
متن کاملInhibition of hepatitis B virus replication by APOBEC3G.
The cytidine deaminase APOBEC3G (A3G) was recently identified as a natural resistance gene that restricts efficient propagation of human immunodeficiency virus and other retroviruses. The enzyme induces massive cytidine to uridine (C3U) deamination of single-stranded retroviral DNA, resulting in DNA degradation or lethal guanine to adenine (G3A) hypermutation (1). Hepadnaviruses, including hepa...
متن کاملAPOBEC3G Inhibits Elongation of HIV-1 Reverse Transcripts
APOBEC3G (A3G) is a host cytidine deaminase that, in the absence of Vif, restricts HIV-1 replication and reduces the amount of viral DNA that accumulates in cells. Initial studies determined that A3G induces extensive mutation of nascent HIV-1 cDNA during reverse transcription. It has been proposed that this triggers the degradation of the viral DNA, but there is now mounting evidence that this...
متن کاملAPOBEC3G and APOBEC3F Require an Endogenous Cofactor to Block HIV-1 Replication
APOBEC3G (A3G)/APOBEC3F (A3F) are two members of APOBEC3 cytidine deaminase subfamily. Although they potently inhibit the replication of vif-deficient HIV-1, this mechanism is still poorly understood. Initially, A3G/A3F were thought to catalyze C-to-U transitions on the minus-strand viral cDNAs during reverse transcription to disrupt the viral life cycle. Recently, it was found more likely that...
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ژورنال
عنوان ژورنال: Retrovirology
سال: 2008
ISSN: 1742-4690
DOI: 10.1186/1742-4690-5-72